To establish the influence of CARP on cardiac hypertrophy in vitro, we taken care of Advert-CARP-GFP- and Advert-GFP-contaminated cells, as properly as non-infected cells (handle), with phenylephrine (a hundred mM) for 24 hrs to induce cardiac hypertrophy
Total RNA was extracted from ventricular tissue and cultured neonatal rat cardiomyocytes making use...