Product Name :
Temporin A peptide
Sequence Shortening :
FLPLIGRVLSGIL
Sequence :
H-Phe-Leu-Pro-Leu-Ile-Gly-Arg-Val-Leu-Ser-Gly-Ile-Leu-OH
Length (aa) :
13
Peptide Purity (HPLC) :
95.3%
Molecular Formula :
C68H116N16O15
Molecular Weight :
1396.8
Source :
Synthetic
Form :
Powder
Description :
C-terminal amidated. Active against B. megaterium Bm11, S. aureus Cowan1, Y. pseudotubercolosis, S. pyogenes beta hem. group A (MIC 1-2.3 uM), E. coli D21 (MIC 11.9 uM), and C. albicans (MIC 3.4 uM); also S. aureus (MIC ~3-8 uM), E. coli (MIC 6.2-100 uM), and A. baumannii (MIC 24 uM). It is also active against channel catfish virus (CCV) as well as the non-enveloped frog virus 3 (FV3) (Chinchar VG et al. Virol. 323:268-275). For NMR studies in SDS and DPC micelles, see Carotenuto et al. 2008 (J Med Chem 51: 2354-62). Transgenic plants: The small, naturally occurring antimicrobial cationic peptide, temporin A, was N-terminally modified (MsrA3) and expressed in potato plants. MsrA3 conveyed strong resistance to late blight and pink rot phytopathogens in addition to the bacterial pathogen Erwinia carotovora. Transgenic tubers remained disease-free during storage for more than 2 years (Transgenic Res. 2004 Apr;13(2):181-90). Updated 3/2015. Temporin A has Antibacterial activity. Temporin A was found in European common frog, Rana temporaria.
Storage Guidelines :
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
References :
Simmaco M, Mignogna G, Canofeni S, Miele R, Mangoni ML, Barra D. Temporins, antimicrobial peptides from the European red frog Rana temporaria. Eur J Biochem. 1996;242(3):788-92.
About TFA salt :
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process. TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product. TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations. In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
Related websites: https://www.medchemexpress.com/peptides/Peptide_Protein.html
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