Product Name :
[Ala11,D-Leu15]-Orexin B peptide
Sequence Shortening :
H-RSGPPGLQGRAQRLLQASGNHAAGILTM-NH2
Sequence :
H-Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Ala-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2
Length (aa) :
28
Peptide Purity (HPLC) :
95.94%
Molecular Formula :
C120H206N44O35S
Molecular Weight :
2857.24
Source :
Synthetic
Form :
Powder
Description :
[Ala11,D-Leu15]-Orexin B is a highly potent and selective OX2 receptor agonist with 400-fold selectivity for OX2 over OX1 receptors, possessing EC50 values of 0.13 and 52 nM for human OX2 and OX1 receptors respectively. [Ala11,D-Leu15]-Orexin B increases social novelty seeking and can decrease anxious and depressive behaviour in animal models.
Storage Guidelines :
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
References :
Asahi et al (2003) Development of an orexin-2 receptor selective agonist, [Ala11D-Leu15]orexin-B. Bioorg.Med.Chem.Lett. 13 111 PMID: 12467628 Borgland et al (2008), Orexin B/hypocretin 2 increases glutamatergic transmission to ventral tegmental area neurons. European J. Neuroscience 28 1545 PMID: 18793323 Staton et al (2018) Orexin 2 receptor stimulation enhances resilience, while orexin 2 inhibition promotes susceptibility, to social stress, anxiety and depression, Neuropharmacology 143 79 PMID: 30240784
About TFA salt :
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process. TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product. TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations. In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
Related websites: https://www.medchemexpress.com/peptides/Peptide_Protein.html
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