He terminal arbors (arrows) was the neuropil. A fine network of labeled axons was discovered within the MdRF,just dorsal for the IO (Figures F,G). Almost all of the terminal boutons were positioned in the neuropil,andwere not associated with counterstained somata. Fewer terminals were present on the contralateral (Figure G) than the ipsilateral side (Figure F). To make sure that the pattern of label seen with all the BDA injections was not as a result of fiberofpassage uptake,we also performed PhaL injections,as this tracer shows small fiberofpassage uptake (Gerfen and Sawchenko. In the illustrated case,the PhaL injection in to the left cMRF was centered within the region,and integrated considerably of its dorsoventral extent (Figures A,B). It extended into the pretectum. In the rostral midbrain,PhaL labeled cMRF axon terminals (stipple) were located bilaterally,with an ipsilateral predominance. Their pattern of termination (Figures A was extremely equivalent to that observed using the BDA injections (Figure. Inside the pons,big numbers of terminals have been found within the PRF (Figures B. Far more axons and terminal boutons were located on the ipsilateral side,as well as the density of termination decreased,and became extra medially concentrated at much more caudal levels. Notably significantly less evident was the labeled fiber track located within a paramedian position on the contralateral side following the BDA injection. This suggests this track mainly represented predorsal bundle axons labeled by way of fiberofpassage uptake of BDA. Terminals have been present all through RIP (Figures F. Fewer labeledFrontiers in Neuroanatomy www.frontiersin.orgApril Volume ArticleWang et al.A cMRF Tectoreticuloreticular PathwayFIGURE Distribution of cMRF terminals labeled by Phaseolus vulgaris leucoagglutinin (PhaL). The distribution of labeled terminals (stipple) and axons (lines) observed following a PhaL injection into the cMRF (A,B) is charted on a rostral to caudal series of sections through the midbrain (A,pons (A and medulla (H.terminals were observed inside the medulla (Figures H,I) than inside the pons,but the ipsilateral predominance was nevertheless evident. The pattern of axonal labeling following this PhaL injection is shown in greater detail in an illustration of a section in the amount of the abducens nucleus (Figure. Note the fairly substantial labeling within the supragenual area (SG) above thefacial nerve. There had been several terminal arbors within both abducens nuclei,however they were scattered. The main terminal field was within the ipsilateral PRF. This field was densest medially,inside the PPRF. Labeled terminal arbors had been also present contralaterally,but they have been considerably fewer in number. The labeling inside the MdRF was consistent using the BDA situations (Figure.Frontiers in Neuroanatomy www.frontiersin.orgApril Volume ArticleWang et al.A cMRF Tectoreticuloreticular PathwayFIGURE PhaL labeled reticuloreticular axons at the degree of the abducens nucleus. The pattern of reticuloreticular axons (lines) and terminals (stipple) is illustrated for the area shown by a box inside the inset. The injection web site is illustrated in Figure . Note the ipsilateral predominance on the terminal labeling within the PRF and MdRF.Photomicrographs showing examples of PhaL labeled terminal arbors in the level of the caudal abducens nucleus (Figure A) are presented in Figures B . Thin,PhaL labeled axons could be observed with occasional branch points and terminal arbors inside this region. The quite a few PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20972551 en passant and terminal boutons (??)-SKF-38393 hydrochloride varied in diameter. The density o.