.3 0.28 six 0.06m# 1.28 six 0.35 37.2 six four.3 38.0 six  23388095 3.7 30.two six five.0 20.four 6 4.3 1.five 6 0.3 34.eight six four.3 20.7 six 4.1 1.7 6 0.three 0.54 6 0.ten 0.73 six 0.21 37.4 six four.two 41.2 six 3.9 ,0.001 NS,0.001,0.001 NS NS NS 0.45 6 0.06 0.74 six 0.17 35.7 six 3.six 38.8 six 3.4 44.five 6 four.eight 25.three six five.two 1.eight 6 0.four 52.4 6 eight.four 26.eight 6 four.6 2.0 6 0.five 0.46 six 0.09 1.34 6 0.35 38.5 6 four.two 41.four six four.6 ,0.001 NS NS,0.001 NS NS NS 0.41 six 0.07 1.27 six 0.29 36.8 six four.six 39.four six four.2 55.7 six 14.0 30.3 6 four.7 1.9 six 0.five 0.26 six 0.11m
.3 0.28 six 0.06m# 1.28 six 0.35 37.2 six four.3 38.0 six 23388095 3.7 30.two six five.0 20.four 6 4.3 1.five 6 0.3 34.eight six four.3 20.7 six 4.1 1.7 6 0.three 0.54 6 0.ten 0.73 six 0.21 37.4 six four.two 41.2 six 3.9 ,0.001 NS,0.001,0.001 NS NS NS 0.45 6 0.06 0.74 six 0.17 35.7 six 3.six 38.8 six 3.4 44.five 6 four.eight 25.three six five.two 1.eight 6 0.four 52.4 6 eight.four 26.eight 6 four.6 2.0 6 0.five 0.46 six 0.09 1.34 6 0.35 38.5 6 four.two 41.four six four.6 ,0.001 NS NS,0.001 NS NS NS 0.41 six 0.07 1.27 six 0.29 36.8 six four.six 39.four six four.2 55.7 six 14.0 30.3 6 four.7 1.9 six 0.five 0.26 six 0.11m

.3 0.28 six 0.06m# 1.28 six 0.35 37.2 six four.3 38.0 six 23388095 3.7 30.two six five.0 20.four 6 4.3 1.five 6 0.3 34.eight six four.3 20.7 six 4.1 1.7 6 0.three 0.54 6 0.ten 0.73 six 0.21 37.4 six four.two 41.2 six 3.9 ,0.001 NS,0.001,0.001 NS NS NS 0.45 6 0.06 0.74 six 0.17 35.7 six 3.six 38.8 six 3.4 44.five 6 four.eight 25.three six five.two 1.eight 6 0.four 52.4 6 eight.four 26.eight 6 four.6 2.0 6 0.five 0.46 six 0.09 1.34 6 0.35 38.5 6 four.two 41.four six four.6 ,0.001 NS NS,0.001 NS NS NS 0.41 six 0.07 1.27 six 0.29 36.8 six four.six 39.four six four.2 55.7 six 14.0 30.3 6 four.7 1.9 six 0.five 0.26 six 0.11m

.three 0.28 6 0.06m# 1.28 six 0.35 37.two 6 four.three 38.0 six three.7 30.two six 5.0 20.four 6 4.three 1.five six 0.three 34.eight six 4.three 20.7 six 4.1 1.7 6 0.3 0.54 6 0.10 0.73 six 0.21 37.4 six 4.two 41.two six three.9 ,0.001 NS,0.001,0.001 NS NS NS 0.45 six 0.06 0.74 six 0.17 35.7 six 3.six 38.8 6 3.4 44.five six 4.8 25.3 six five.2 1.8 6 0.4 52.four six 8.4 26.eight six four.6 two.0 six 0.five 0.46 6 0.09 1.34 six 0.35 38.five six four.2 41.four 6 four.6 ,0.001 NS NS,0.001 NS NS NS 0.41 6 0.07 1.27 6 0.29 36.eight 6 4.six 39.4 6 4.2 55.7 six 14.0 30.three 6 4.7 1.9 six 0.5 0.26 six 0.11m 1.81 six 0.67 35.four six 4.5 35.8 six 3.8 59.9 six 14.6 31.four six four.0 1.9 six 0.five 58.9 6 11.three 29.eight six five.0 two.1 6 0.six 0.45 6 0.11 1.58 6 0.55 39.0 six four.9 41.three 6 5.5 NS NS NS,0.001 NS NS 0.049 0.39 6 0.10 1.72 6 0.68 35.64 six 4.eight 38.0 six four.2 Data are presented as indicates 6 SD; VE = ventilation; RR = respiratory price; VT = tidal volume; VD = dead space volume; VCO2 = carbon dioxide production; PaCO2 = arterial carbon dioxide stress; PETCO2 = End-tidal carbon dioxide stress; bpm = breaths per minute; $: p,0.05 vs. 250 mL; m: p,0.001 vs. 500 mL; : p,0.001 vs. 250 mL; &: p,0.01 vs.500 mL; #,0.01 vs. 250 mL. doi:ten.1371/journal.pone.0087395.t003 primitive chemoreceptor abnormalities as drivers of the alveolar hypoventilation observed in COPD patients. Thirdly, with the Yintercept we analyze an index of overall DS. However, in the present setting, we were able to change DS only by adding an external DS, so that we do not know if changes in MedChemExpress GHRH (1-29) physiological DS similarly influence the VEYint. Fourthly, VE changes during exercise are due to VCO2, VD/ VT and PaCO2 changes, and all may influence the VE vs. VCO2 relationship. In the present study, we added external DS, which at each step of exercise, was associated to an increase of VD/VT and PaCO2 resembling what happens during exercise in COPD patients. Therefore both PaCO2 and VD/VT changes have likely a role in the VE vs. VCO2 relationship changes we observed after adding DS. It is recognized that PaCO2 measurements were done only in HF patients and not in healthy subjects, but a different behaviour in healthy subjects is unlikely. Fifthly, the condition of VE at CO2 production equal 0, as such at the VEYint of the VE vs. VCO2 relationship, is a mathematical extrapolation with no physiological meaning. Moreover, absolute DS changes during exercise, so that also the VEYint value is likely close but different from the rest value. Indeed, we showed that VD tended to increase in HF patients and to reduce in healthy subjects during exercise without added DS. However, we 1418741-86-2 suggest using VEYint as a tool to evaluate the presence of an increased DS, regardless of its physiological meaning with respect to rest and exercise. The adding of DS significantly reduced the external work produced in HF patients, while a not significant reduction was observed in normal subjects. Peak VO2 remained unchanged in both groups after adding DS; this finding suggests that added DS was associated to an increased work of breathing which, as a percentage of total work, seems to be greater in HF patients than in normal subjects. Estimation of Dead Space Ventilation HEART FAILURE PATIENTS ADDED DEAD SPACE +0 mL +250 mL 2865 9.6962.91 1563 1 ANOVA p value +500 mL 2964 13.2663.18 1663 1 VE/VCO2 slope VEYint RRYint VDYint VDmeas HEALTHY SUBJECTS VE/VCO2 slope VEYint RRYint VDYint VDmeas 2764 4.9861.63, construct consecutive coarse-grained time series,, determined by the scale factor, t, jt 1 X N Xi, 1j. For according to the equation: y ~ j t i~tz1 t scale one, the time series is simply the ori..three 0.28 six 0.06m# 1.28 6 0.35 37.two six 4.3 38.0 6 3.7 30.2 six 5.0 20.four 6 four.three 1.five six 0.three 34.eight six 4.three 20.7 6 4.1 1.7 6 0.3 0.54 six 0.ten 0.73 6 0.21 37.4 6 four.two 41.2 6 3.9 ,0.001 NS,0.001,0.001 NS NS NS 0.45 6 0.06 0.74 six 0.17 35.7 six 3.6 38.eight six three.4 44.five six four.8 25.three six five.two 1.8 six 0.4 52.four 6 8.4 26.eight 6 4.six 2.0 6 0.5 0.46 6 0.09 1.34 six 0.35 38.five 6 4.two 41.4 six four.six ,0.001 NS NS,0.001 NS NS NS 0.41 six 0.07 1.27 6 0.29 36.8 6 four.6 39.4 six 4.two 55.7 six 14.0 30.three six four.7 1.9 6 0.five 0.26 six 0.11m 1.81 six 0.67 35.four six 4.five 35.8 6 three.eight 59.9 six 14.6 31.4 6 4.0 1.9 6 0.5 58.9 six 11.3 29.eight six five.0 2.1 6 0.6 0.45 6 0.11 1.58 six 0.55 39.0 six four.9 41.three 6 5.five NS NS NS,0.001 NS NS 0.049 0.39 6 0.10 1.72 6 0.68 35.64 six 4.8 38.0 6 four.2 Information are presented as signifies 6 SD; VE = ventilation; RR = respiratory price; VT = tidal volume; VD = dead space volume; VCO2 = carbon dioxide production; PaCO2 = arterial carbon dioxide pressure; PETCO2 = End-tidal carbon dioxide pressure; bpm = breaths per minute; $: p,0.05 vs. 250 mL; m: p,0.001 vs. 500 mL; : p,0.001 vs. 250 mL; &: p,0.01 vs.500 mL; #,0.01 vs. 250 mL. doi:10.1371/journal.pone.0087395.t003 primitive chemoreceptor abnormalities as drivers of the alveolar hypoventilation observed in COPD patients. Thirdly, with the Yintercept we analyze an index of overall DS. However, in the present setting, we were able to change DS only by adding an external DS, so that we do not know if changes in physiological DS similarly influence the VEYint. Fourthly, VE changes during exercise are due to VCO2, VD/ VT and PaCO2 changes, and all may influence the VE vs. VCO2 relationship. In the present study, we added external DS, which at each step of exercise, was associated to an increase of VD/VT and PaCO2 resembling what happens during exercise in COPD patients. Therefore both PaCO2 and VD/VT changes have likely a role in the VE vs. VCO2 relationship changes we observed after adding DS. It is recognized that PaCO2 measurements were done only in HF patients and not in healthy subjects, but a different behaviour in healthy subjects is unlikely. Fifthly, the condition of VE at CO2 production equal 0, as such at the VEYint of the VE vs. VCO2 relationship, is a mathematical extrapolation with no physiological meaning. Moreover, absolute DS changes during exercise, so that also the VEYint value is likely close but different from the rest value. Indeed, we showed that VD tended to increase in HF patients and to reduce in healthy subjects during exercise without added DS. However, we suggest using VEYint as a tool to evaluate the presence of an increased DS, regardless of its physiological meaning with respect to rest and exercise. The adding of DS significantly reduced the external work produced in HF patients, while a not significant reduction was observed in normal subjects. Peak VO2 remained unchanged in both groups after adding DS; this finding suggests that added DS was associated to an increased work of breathing which, as a percentage of total work, seems to be greater in HF patients than in normal subjects. Estimation of Dead Space Ventilation HEART FAILURE PATIENTS ADDED DEAD SPACE +0 mL +250 mL 2865 9.6962.91 1563 1 ANOVA p value +500 mL 2964 13.2663.18 1663 1 VE/VCO2 slope VEYint RRYint VDYint VDmeas HEALTHY SUBJECTS VE/VCO2 slope VEYint RRYint VDYint VDmeas 2764 four.9861.63, construct consecutive coarse-grained time series,, determined by the scale factor, t, jt 1 X N Xi, 1j. For according to the equation: y ~ j t i~tz1 t scale one, the time series is simply the ori.