Quantitation of the mutant Vp1s and actin in HeLa cells transfected with the Vp1 expression plasmids or with an vacant vector (Mock) were being analyzed by SDS-Website page and immunoblotting for Vp1 (Vp1) and for actin (act)
Mutations at the cysteine residues in the Vp1 coding phase of pUC19-Mad1SVED had been...