Gle product. Extending the overall exposure time should result in a more extensive conversion. SUMMARY 1-Ethynyl-dSpacer, in combination with psoralen and coumarin azides, has been shown to be a potential research tool for studying ICL. As a simple Click tool, 1-Ethynyl-dSpacer works exceptionally well to introduce a 1,2,3-substitutedtriazole residue into an oligonucleotide as a pseudo nucleobase, while preserving the natural phosphodiester backbone. With the selection of a suitable azide such as psoralen or coumarin, the modifier can be used to incorporate photo-inducible 8
and site-specific ICL. The versatility of 1-Ethynyl-dSpacer is highlighted by the fact that a single 1 ole synthesis can be used with multiple azides to generate and evaluate several different structures.
CHOLESTERYL-TEG PHOSPHORAMIDITES AND SUPPORT NOW CERTIFIED BSE/TSE FREE
Cholesterol remains one of the most popular forms of labelling of oligonucleotides, especially those destined for use in therapeutics, including antisense and siRNA. 1,2 Most oligonucleotides are hydrophilic, so they are constantly challenged in their ability to permeate cell membranes. In order to improve cellular uptake, one strategy is to conjugate oligonucleotides with hydrophobic molecules that are nontoxic and cholesterol is one of the most popular choices. In 1993, Glen Research introduced Cholesteryl-TEG phosphoramidite (1) and the corresponding CPG support (2). Later, in 2008, we introduced the simplified version 5′-Cholesteryl-TEG phosphoramidite (3), designed solely for 5′ labelling of oligonucleotides.301353-96-8 manufacturer In those early days, the cholesterol to make these products was sourced from sheep’s wool and the products were perfectly useable for their intended research and development purposes. However, it has become clear that this cholesterol source was unacceptable in situations where product oligonucleotides were required to be certified as being free from BSE/ TSE contamination. Bovine spongiform encephalopathy (BSE) is a transmissible spongiform encephalopathy (TSE) that affects cattle. There is also a spongiform encephalopathy associated with sheep, called scrapie, that has been shown to induce BSE in cattle. We are happy to report that our raw material cholesterol is now from a plant source and our cholesterol products can now be certified as free from BSE/TSE contamination. Glen Research also offers other hydrophobic labels, 5′-a-TocopherolTEG Phosphoramidite (4) and 5′-Stearyl Phosphoramidite (5) , to improve the cellular uptake of oligonucleotides. REFERENCES:
1. M. Raouane, D. Desmaele, G. Urbinati, L. Massaad-Massade, and P. Couvreur, Bioconjugate Chemistry, 2012, 23, 10911104.219989-84-1 manufacturer 2.PMID:25905319 J. Winkler, Ther Deliv, 2013, 4, 791-809. FIGURE 1: STRUCTURES OF CHOLESTERYL AND LIPOPHILIC PHOSPHORAMIDITES AND SUPPORT
NEW PRODUCT – DEPURINATION RESISTANT dA-CE PHOSPHORAMIDITE
INTRODUCTION Depurination is defined as the cleavage of the glycosidic bond attaching a purine base to the sugar moiety. Electron withdrawing acyl protecting groups like benzoyl and isobutyryl on the purine amino group(s) destabilize the glycosidic bond, whereas electron donating formamidine protecting groups stabilize the glycosidic bond. The use of formamidine protecting groups to eliminate depurination was first proposed1,2 in the 1980s and dmf-dG is an example of this strategy in routine use today. The consequence of depurination during oligonucleotide synthesis is the loss of the purine base to form an internucleotide linka.MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com
