Name :
UBE2D1 Protein
Description :
Ubiquitin-conjugating enzyme E2 D1 (UBE2D1), a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, which is short for stimulator of iron (Fe) transport. In other words, UbcH5A is significantly up-regulated in the liver of iron-overloaded patients with hereditary hemochromatosis, as previously published for SFT. Moreover, a complex of UBE2D1 is critical in maintaining KRAS protein stability and propose that targeting such complex may be a unique strategy to degrade mutant KRAS to kill cancer cells.
Species :
Human
Uniprotkb :
E. coli
Tag :
His
Synonyms :
UBC4/5, E2(17)KB1, ubiquitin-conjugating enzyme E2D 1, UBCH5, UBCH5A, SFT
Construction :
A DNA sequence encoding the human UBE2D1 (P51668) (Ala 2-Met 147) was expressed, with a polyhistide tag at the N-terminus.
Protein Purity :
> 90 % as determined by SDS-PAGE
Molecular Weight :
Approxiamtely 18.3 kDa
Endotoxin :
Please contact us for more information.
Formulatione :
Supplied as sterile 25mM Tris, 100mM NaCl, 20% glycerol, 0. 05% TweenPlease contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution :
A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage :
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping :
Solution. It is shipped out with blue ice.
Research Background :
Ubiquitin-conjugating enzyme E2 D1 (UBE2D1), a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, which is short for stimulator of iron (Fe) transport. In other words, UbcH5A is significantly up-regulated in the liver of iron-overloaded patients with hereditary hemochromatosis, as previously published for SFT. Moreover, a complex of UBE2D1 is critical in maintaining KRAS protein stability and propose that targeting such complex may be a unique strategy to degrade mutant KRAS to kill cancer cells.
References and Literature :
1. Zhang L,et al.(2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor. Genes Dev. 25(12): 1262-74. 2. Tokumoto M,et al.(2011) Cadmium toxicity is caused by accumulation of p53 through the down-regulation of Ube2d family genes in vitro and in vivo. J Toxicol Sci. 36(2): 191-200. 3. Ohbayashi N,et al.(2008) Physical and functional interactions between ZIP kinase and UbcH5. Biochem Biophys Res Commun. 372(4): 708-12. 4. Gehrke SG,et al.(2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis. Blood. 101(8): 3288-93.
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