mouse anti-V5 tag monoclonal antibody (V5.E10) 7678

Additiona Information:
9 5 2 inhostmouse|isotypeIgG1|clonalitymonoclonal|concentration1 mg/mL|applicationsELISA, ICC/IF, WB|reactivitytagged fusion proteins|available sizes1 mg, 100 g, 25 g|mouse anti-V5 tag monoclonal antibody (V5.E10) 7678antibodyDatabase link:V5 tagSequence – GKPIPNPLLGLDSTTested applicationsWB,ICC/IF,ELISARecommended dilutionsWB: 1:1000-3000ICC/IF: 1:500-2000For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test.ImmunogenGKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLHSize and concentration25, 100, 1000g and 1 mg/mLFormliquidStorage bufferPBS, pH 7.2, 0.05% NaN3Purityaffinity purifiedClonalitymonoclonalIsotypeIgG1Compatible secondariesgoat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721Isotype controlMouse monocolonal IgG1 – Isotype Controltarget relevanceV5 tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify V5tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, V5 tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.Click for more on: epitope tagsand V5 tagBiotechnologyThe V5 tag has become a valuable tool in scientific research, particularly in studies involving the expression and detection of recombinant proteins. The V5 tag is a short peptide sequence (GKPIPNPLLGLDST) that can be fused to the N or C terminus of a target protein, allowing for easy detection and purification. Researchers commonly utilize the V5 tag as an epitope for specific V5 tag antibodies, enabling convenient and sensitive visualization of the tagged protein in various experimental assays, including Western blotting and immunofluorescence. The small size of the V5 tag minimizes potential interference with the function and properties of the tagged protein, making it an attractive choice as a molecular tag. Additionally, the V5 tag can be used for protein purification using affinity chromatography, facilitating the isolation and characterization of the tagged protein. The widespread use of the V5 tag in research owes to its efficiency, reliability, and compatibility with various expression systems, making it an essential tool for investigating protein dynamics, interactions, and cellular localization in diverse biological contexts.|

Antibodies are immunoglobulins secreted by effector lymphoid B cells into the bloodstream. Antibodies consist of two light peptide chains and two heavy peptide chains that are linked to each other by disulfide bonds to form a “Y” shaped structure. Both tips of the “Y” structure contain binding sites for a specific antigen. Antibodies are commonly used in medical research, pharmacological research, laboratory research, and health and epidemiological research. They play an important role in hot research areas such as targeted drug development, in vitro diagnostic assays, characterization of signaling pathways, detection of protein expression levels, and identification of candidate biomarkers.
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EZR Antibody In Vivo Pirh2 Antibody Purity PMID:34166303 MedChemExpress (MCE) offers a wide range of high-quality research chemicals and biochemicals (novel life-science reagents, reference compounds and natural compounds) for scientific use. We have professionally experienced and friendly staff to meet your needs. We are a competent and trustworthy partner for your research and scientific projects.Related websites: https://www.medchemexpress.com